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KMID : 0358419940370040756
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Korean Journal of Obstetrics and Gynecology
1994 Volume.37 No. 4 p.756 ~ p.768
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Study on the Establishment and Characterization of CA 125 Producing Cell Line (SCHE-1) Orignating from a Human Endometrial Adenocarcinoma and Modulation of CA 125 Production by Dibuty1 Cyclic Adenosine Monophosphate (dbc
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김순덕
이순곤/남계현/이권해/조태호
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Abstract
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A new humah endometrial carcinoma cell line, designated SCHE-1, was established from the endometrial adenocarcinoma of a 68-year-old woman. This cell line has grown well for 16 months and has been subcultured more than 50 times. Monolayer
cultured
cell
are polygonal in shape, showing a pavement-like arrangement and a piling up tendency without contact inhibition. The chromosomal number shows aneuploidy and the modal chromosomal number is in the diploid rage. 1×10E5 SCHE-1 cells produced CA
125(184-682U)during 19 days in culture media. CA 125 was demonstrated immunohistocytochemically in the original tumor, and SCHE-1 cells. The cells contain no estrogen or progesterone receptors.
We studied the effects of dibutyl cyclic adenosine monophosphate on cellular growth and production of CA125 in vitro. It is possible that the serum in the in vitro culture medium contains CA 125 and influences the activity of dibutyl cyclic
adenosine
monophosphate. Dibutyl cyclic adenosine monophosphate (which is said to have an influence upon gene expression)suppressed their proliferation and increased CA 125 levels.
A dose-response relationship exists between the concentration of abc AMP and CA125 levels in the conditioned media, while the normal cells could not produce even after being administered dibutyl cyclic adenosine monophosphate. The above fact
suggests
that CA 125 gene expression was induced by dibutyl cyclic adenosine monophosphate.
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